Cells control vital bio-chemical processes by creating compartments with distinct compositions. In addition to the well-known membrane-bound organelles, such as the nucleus or chloroplasts, dynamic membraneless compartments formed through liquid-liquid phase separation have emerged as important cellular constituents over the last decades. These biomolecular condensates are liquid-like, their absence of membrane allow easy chemical exchanges with their surroundings, they can coalesce, exert capillary forces and dissolve or condense easily. While their formation and liquid-like properties are well established, much less is known on the effect of biochemical activity on them. In this talk, I will present our artificial chemically active biomolecular condensates based on Bovine Serum Albumin (BSA) rich droplets seeded with enzymes. I will show that the enzymatic activity can generate concentrations gradients that drives Marangoni swimming. Yet, only specific molecules trigger this Marangoni response in our surfactant free system. I will show that the surface tension variations emerge from local perturbations of the thermodynamic phase equilibrium and can be easily summarized as: phase-separated droplets swim to their dissolution. [1] Phase-Separated Droplets Swim to Their Dissolution. E Jambon-Puillet, A Testa, CM Lorenz, RW Style, A Rebane, ER Dufresne. bioRxiv, 2023.07.18.549556 (2023).
Accès Salle des séminaires FAST-LPTMS (Bât. 530, salle C.120, 1er)